Digital holographic microscope

Unlike conventional brightfield microscopes, digital holographic microscopes can capture objects from an entire transparent volume. Therefore, they can monitor even 300 times larger sample volume by taking a single snapshot. At HUN-REN SZTAKI, we have developed three different digital holographic microscope models:

• HiRES: Algae monitoring
  • Typical object size: 3-75 μm
  • Holographic resolution: 0,6 μm
  • Measurement speed: 3,6-18 μl/min
  • Sample depth: 200 μm
• HiRES+Fluor: Algae monitoring with fluorescent extension
  • Typical object size: 3-75 μm
  • Holographic resolution: 0,6 μm
  • Measurement speed: 3,6-18 μl/min
  • Sample depth: 200 μm
  • Fluorescent detection accuracy: 4 μm
  • Excitation wavelength: 445-480nm (changeable)
  • Emission wavelength: >500nm (changeable)
• LVol: Drinking water monitoring
  • Typical object size: 15-300 μm
  • Holographic resolution: 2 μm
  • Measurement speed: 15-50 μl/min
  • Sample depth: 10mm

The holographic microscope can perform automatic operation. Using flow-through chamber, it can automatically capture holograms, detect and classify them using deep learning AI. The built in classification module can be easily retrained.

Application areas:

• Drinking water safety;
• Monitoring algae, worms, and protozoa in different samples;
• Automatized monitoring of Algae cultivation;
• Environment monitoring/protection;
• Monitoring industrial water;
• Monitoring of the products in food industry;
• Monitoring object particle sizes even from multiple aspects (e.g. pharmaceutical industry);
• Ballast water monitoring;
• Measuring cyanobacteria concentration;
• Fishery water quality inspection.

The microscopes can be ordered from Holodetect Instruments Ltd. Pilot project proposals in new application areas are welcome!